(A) Step one 1 of the licensing assay was completed mixing high-speed Xenopus extract at a 1:3 percentage with either Buffer (Buf) or soluble extracts from CHO cells synchronized in the indicated instances following mitosis. of geminin and Cdk actions, … Continue reading (A) Step one 1 of the licensing assay was completed mixing high-speed Xenopus extract at a 1:3 percentage with either Buffer (Buf) or soluble extracts from CHO cells synchronized in the indicated instances following mitosis